Ruxolitinib inhibits transforming JAK2 fusion proteins in vitro and induces complete cytogenetic remission in t(8;9)(p22;p24)/PCM1-JAK2-positive chronic eosinophilic leukemia.

systematic triton lysis as a control to prove the vesicular nature of the MP population, we agree that the use of triton lysis can be a validating step for many MP-IC applications by FCM, as we also saw MP population dissolve from the MP gate. However, using the triton lysis we observed an impact on the IC population (data not shown). Under our conditions, as both populations are distinguishable by light scatter alone, the use of triton lysis in this instance added an unnecessary and complicating step. Here we have shown that MPs and ICs do not share biophysical light scatter properties when analyzed by FCM, but because of the small size of both, and the limits of some FCM instruments, maintenance, and operation, they may not appear discrete.